LOCUS       40924_26466       12861 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Ti-curing vector pK18msr24A DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Puhler A.
  TITLE     Small mobilizable multi-purpose cloning vectors derived from the 
            Escherichia coli plasmids pK18 and pK19: selection of defined 
            deletions in the chromosome of Corynebacterium glutamicum
  JOURNAL   Gene 145 (1), 69-73 (1994)
  PUBMED    8045426
REFERENCE   2  (bases 1 to 12861)
  AUTHORS   Yamamoto S, Uraji M, Tanaka K, Moriguchi K, Suzuki K.
  TITLE     Identification of pTi-SAKURA DNA region conferring enhancement of 
            plasmid incompatibility and stability
  JOURNAL   Genes Genet. Syst. 82 (3), 197-206 (2007)
  PUBMED    17660690
REFERENCE   3  (bases 1 to 12861)
  AUTHORS   Yamamoto S, Kiyokawa K, Tanaka K, Moriguchi K, Suzuki K.
  TITLE     Novel toxin-antitoxin system composed of serine protease and 
            AAA-ATPase homologues determines the high level of stability and 
            incompatibility of the tumor-inducing plasmid pTiC58
  JOURNAL   J. Bacteriol. 191 (14), 4656-4666 (2009)
  PUBMED    19447904
REFERENCE   4  (bases 1 to 12861)
  AUTHORS   Yamamoto S, Suzuki K.
  TITLE     Development of a reinforced Ti-eviction plasmid useful for 
            construction of Ti plasmid-free Agrobacterium strains
  JOURNAL   J. Microbiol. Methods 89 (1), 53-56 (2012)
  PUBMED    22326751
REFERENCE   5  (bases 1 to 12861)
  AUTHORS   Yamamoto S, Suzuki K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Contact:Shinji Yamamoto Hiroshima 
            University, Graduate School of Science; Kagamiyama 1-3-1, 
            Higashi-Hiroshima, Hiroshima 739-8526, Japan
REFERENCE   6  (bases 1 to 12861)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 12861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1994"; volume: "145"; issue: "1"; pages: "69-73"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Genes 
            Genet. Syst."; date: "2007"; volume: "82"; issue: "3"; pages: 
            "197-206"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2009"; volume: "191"; issue: "14"; pages: 
            "4656-4666"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2012"; volume: "89"; issue: "1"; pages: 
            "53-56"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2012) Contact:Shinji Yamamoto Hiroshima University, Graduate
            School of Science; Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima 
            739-8526, Japan"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             346..1137
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        1186..1631
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             1632..3050
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     oriT            complement(3407..3516)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        complement(3751..3841)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(4035..4125)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      4485..5073
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5361..5382
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5397..5427
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5435..5451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5459..5475
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5651..6205
                     /codon_start=1
                     /gene="tiorf24"
                     /product="putative antitoxin of toxin-antitoxin system"
                     /function="involved in plasmid stabilization"
                     /label=tiorf24
                     /protein_id="BAL46490.1"
                     /translation="MGAPLDIFKKVVERAPIKPQLVKRGGRSIRQFGQAELVFLHAYDE
                     LKLALTPKSQSEFYEALQTSLKRGLAKEVVFGKQRYDIGQHLVFVERKLKELEKLTDQV
                     DLSGKEPVIRGTHIEAHRIAALLDAGATVEEVLRDYPSLKEQQVVAARVYAEAHPKAGR
                     PYPKQTAKAAMRAADLSALDD"
     gene            5651..6205
                     /gene="tiorf24"
                     /label=tiorf24
     protein_bind    6473..6486
                     /label=TraR binding site
                     /bound_moiety="TraR"
                     /note="Tra box"
     protein_bind    6564..6577
                     /label=TraR binding site
                     /bound_moiety="TraR"
                     /note="Tra box"
     CDS             6776..7993
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A"
                     /function="involved in vegetative DNA replication"
                     /label=repA
                     /protein_id="BAL46491.1"
                     /translation="MSKAAAISRNDRPSVDVTIGEHAEQLSSQLQAMSEALFPPTSHKS
                     LRKFTSGEAARLMKISDSTLRKMTLAGEGPQPELASNGRRFYTLGQINEIRQMLAGSTR
                     GRESIDFVPHRRGSEHLQVVAVTNFKGGSGKTTTSAHLAQYLALQGYRVLAVDLDPQAS
                     LSALLGVLPETDVGANETLYAAIRYDDTRRPLRDVIRPTYFDGLHLVPGNLELMEFEHT
                     TPKALTDKGTRDGLFFTRVAQAFDEVADDYDVVVIDCPPQLGFLTLSGLCAATSMVITV
                     HPQMLDIASMSQFLLMTRDLLGVVKEAGGNLQYDFIRYLLTRYEPQDAPQTKVTALLRN
                     MFEDHVLTNPMVKSAAVSDAGLTKQTLYEIGRENLTRSTYDRAMESLDAVNSEIEALIK
                     MAWGRV"
     gene            6776..7993
                     /gene="repA"
                     /label=repA
     CDS             8223..9233
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B"
                     /function="involved in vegetative DNA replication"
                     /label=repB
                     /protein_id="BAL46492.1"
                     /translation="MSRKDAIDTLFLKKQPATDRAAVDKSTARVRTGAISAMGSSLQEM
                     AEGAKAAARLQDQLATGEAVVSLDPSMIDGSPIADRLPSDVDPKFEQLEASISQEGQQV
                     PVLVRPHPEAAGRYQIVYGRRRLRAAVNLRREVSAIVRNLTDCELVVAQGRENLNRADL
                     SFIEKALFALRLEDAGFDRATIIAALSTDKADLSRYITVARGIPLNLATQIGPASKAGR
                     SRWVALAEGLGKPKATDAIEAMLGSEQFKQSDSDTRFNLIFNAVSRPPAKTPKKVRAWS
                     TPKGKKAATIRQETGRTALVFDERLVPTFGEYVADQLDSLYAQFIETNGGGKLDQ"
     gene            8223..9233
                     /gene="repB"
                     /label=repB
     rep_origin      9288..9446
                     /label=pTi-SAKURA oriV
                     /note="pTi-SAKURA oriV"
     CDS             9448..10767
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C"
                     /function="involved in vegetative DNA replication"
                     /label=repC
                     /protein_id="BAL46493.1"
                     /translation="MQTHISTTSFGRRPMTLGHIASQMAAKAVASDTVAHKWQVFQHIR
                     ESRGLIGATDRSLSILNALLTFYPETALTGGAELVVWPSNEQLMARANGMPATTLRRHL
                     AILVDCGLIIRRDSPNGKRFARKGRGGEIEQAYGFDLSPIVARAEEFRDLAQTVQAEKK
                     AFRVAKERLTLLRRDIVKMIETGVEESVPGNWGRVTQTYQGIIGRLPRSAPRQLVESIG
                     QELQELCIEIRDVLESFTKTMNLDANESHIGRHKQNSNPDSKFESEYGSGKKDEAGGSV
                     AETDNVRSLPKRELPLGIVLDACPEMRELAQGGPIRHWRDLLAAAELARPMLGISPSAW
                     REARETMGEQHAAITLASIYQRAGQINNAGGYLRSLTDRAKDGKFSTWPMVMALLRAKL
                     DEQKNAVGAGKPRTAEEVEDDSRLHVSESLLKNLRKPRSW"
     gene            9448..10767
                     /gene="repC"
                     /label=repC
     primer_bind     complement(11274..11290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11732..12769
                     /codon_start=1
                     /gene="ietA"
                     /product="AAA-family ATPase"
                     /function="involved in plasmid stabilization"
                     /label=ietA
                     /protein_id="BAL46494.1"
                     /translation="MDHSIRRSSIYEPSSVVMTTAQQLISLLKSHVEGDDEQFLTIALQ
                     AAASEARRGHGKVAVQLRELVDAARANKDRTSGRKKAPVPIAQPRGDLANLVAVRYTDV
                     RLSSMILPAELEARLKRVILEQRQQHKLRSHGLAPRRKLLLIGPPGSGKTMTAAALAGE
                     LHTPLFTVQLDGLMTKFMGETASKLRLVFSAMAETKGVYFFDEFDAIGAKRAERNDVGE
                     IRRVLNSFLQFLEEDESDGLVVAATNHPELLDPALFRRFDDVIEYALPSVEVATGILQA
                     RLSGFDTQDIDWTKAVADAAGLSQAEIARAAADAAKLVVLSDRDRITQNDLSLAIAERK
                     GAASQ"
     gene            11732..12769
                     /gene="ietA"
                     /label=ietA
ORIGIN
        1 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
       61 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
      121 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
      181 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
      241 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
      301 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
      361 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      421 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      481 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
      541 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      601 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      661 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      721 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      781 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      841 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
      901 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      961 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     1021 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1081 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     1141 gcgggactct ggggttcgct agaggatcga tcctttttaa cccatcacat atacctgccg
     1201 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
     1261 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
     1321 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
     1381 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
     1441 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
     1501 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
     1561 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
     1621 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
     1681 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
     1741 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
     1801 agcaaaaaaa tgaaaaatat caagtttctg aatttgattc gtccacaatt aaaaatatct
     1861 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg
     1921 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg
     1981 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga
     2041 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa
     2101 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt
     2161 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag
     2221 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa
     2281 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact
     2341 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca
     2401 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt
     2461 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac
     2521 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg
     2581 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag
     2641 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca
     2701 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc
     2761 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg
     2821 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac
     2881 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg
     2941 cagacaaaca atcaacgttt gcgccgagct tcctgctgaa catcaaaggc aagaaaacat
     3001 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca
     3061 aaagaaaatg ccgatgggta ccgagcgaaa tgaccgacca agcgacgccc aacctgccat
     3121 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     3181 gggacgccct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
     3241 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
     3301 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
     3361 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
     3421 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
     3481 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
     3541 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
     3601 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc
     3661 cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac
     3721 tgaggggaca ggcgagagac gatgccaaag agctcctgaa aatctcgata actcaaaaaa
     3781 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     3841 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     3901 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
     3961 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
     4021 cttctgtttc tatcagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     4081 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     4141 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
     4201 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac
     4261 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     4321 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaaaagg
     4381 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5281 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5341 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5401 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     5461 ggaaacagct atgacatgat tacgaattcg agctcggtac ccggggatcc tctagatatt
     5521 gtctgaaaca ccctgccaaa atgaaatcaa acgggggcct tgcactatct ctaattcggc
     5581 cctatctttc ggggtgaatt agagaggcga tcggatggaa catctgacaa cggcacaggc
     5641 ggcttttgta gtgggcgcgc cgctggacat cttcaagaag gttgtcgagc gcgcgccgat
     5701 taagccgcaa ctcgtgaagc gaggtggtcg aagcattcga cagttcggtc aagctgagct
     5761 ggtgtttctc catgcctatg acgaactcaa actggcgctc acgcccaaga gccaatccga
     5821 gttctatgag gcgttgcaga cctccctaaa gcgcggtctc gcgaaggaag tcgtgttcgg
     5881 caaacagcgc tacgacatcg gtcagcactt agtcttcgtc gagcgcaagc tgaaggaact
     5941 cgagaagctc accgatcagg tcgacctatc cgggaaggag ccggtcatcc gaggcacgca
     6001 catcgaggct catcggatcg ctgctcttct cgacgccggc gccacggtcg aagaggtcct
     6061 tcgcgactat ccctccttga aggaacaaca ggtcgtggcg gcgcgagttt atgcggaggc
     6121 gcatccgaag gcgggccggc cgtacccgaa gcaaacggca aaagccgcca tgcgcgcagc
     6181 cgatcttagc gcgctggatg actgagcttg aaatacctgc tcgatacaaa tgtcctgaaa
     6241 gagattggtc gacccgaacc gcacgagaat gtcgcggctt ggctcgatac gatcgatgat
     6301 accgatctcg ccatcagcgt gatctcggtt cgagagattc tagagtcgac ctgcaggcat
     6361 gcaagcttcg ataccgttcg tattggtccg gcgaaactgt gagtatccgc atcgtaatct
     6421 ccgcatgaac aggtcatgcg aacagaaatc atctcacggt gcgtttgcct acgtgcagat
     6481 ttgcacctca ggtgattcta ccgagtcggt gttccaaggc gcgaaatgcg agcgggtgag
     6541 gccggccaga cgccgacaag gttgtgcaga tctgcacttg gtgccacgtc gcacagaaga
     6601 agggaatcgg tctaactcac agatagcatt tgaagaatcg ggatttagtg tgatttcgat
     6661 tgaaacgcgc gtaaccgttc attaaccaaa aacgtcttgc aacctcaccc gcattaggta
     6721 atcgtcacgg ataaatggca atacgcgcca attaaccgtg acaagagata acaccgtgag
     6781 caaagccgct gccatatccc gaaatgatcg cccgtcggta gatgttacca ttggtgagca
     6841 tgctgagcag ctcagctctc agcttcaagc gatgagcgag gctttgtttc ctccgacgtc
     6901 gcacaagagc ttgcgcaaat tcacctcggg tgaagccgca cgcttgatga aaatatctga
     6961 ctcaactctt cgaaagatga cactggctgg cgaagggccg caacctgaac tcgccagcaa
     7021 cggacggcgc ttttacaccc tcggtcagat aaacgaaatc cggcagatgc ttgccggctc
     7081 gactcgagga cgtgaaagca ttgattttgt gcctcatcgc cgaggttctg agcatttgca
     7141 agtcgttgct gtaaccaact tcaaaggtgg ctctgggaag acgacgacgt ccgctcatct
     7201 tgcacagtat ctggcgttgc aaggttacag ggttctcgca gtcgatctcg atccgcaggc
     7261 tagtctttca gcactcctcg gcgttctgcc agaaactgat gtcggtgcaa acgaaacgct
     7321 ctatgcggct attcggtacg acgacacacg tcgtccgttg cgagatgtga tccgaccgac
     7381 gtattttgat ggtcttcacc ttgttcctgg aaatctcgag cttatggagt tcgagcatac
     7441 caccccgaaa gcattgactg acaaaggtac gcgcgacgga ttgttcttca ctcgcgtggc
     7501 ccaagccttt gatgaggtcg ccgacgatta cgatgtcgtg gtcatcgact gccctcctca
     7561 gcttgggttt ttgactctca gcgggttgtg tgctgcaaca tcaatggtaa tcaccgtaca
     7621 tcctcagatg ctggatatcg cttccatgag ccagtttctc ctcatgacac gcgaccttct
     7681 gggtgtcgtg aaagaggcgg ggggcaatct ccagtacgat ttcatacgct atctcttgac
     7741 gcgctatgag ccccaggacg cgccgcagac gaaagtgacg gcactgctgc gcaacatgtt
     7801 cgaggatcac gtccttacaa atcctatggt caagtcggca gcggtatctg atgccggttt
     7861 aaccaagcag acgctctatg agatagggcg agagaacctt acgcgatcga catacgaccg
     7921 ggcgatggaa tctttagatg cggtgaattc ggagatcgag gctttgatca agatggcgtg
     7981 ggggcgggtc taatgaaagg ctttgcgttc ctcacagatc tgttgggagc tcccaacaga
     8041 caggtgttga ttcgccccct ggacatgggg cactggagaa gccggggtaa tttgagacga
     8101 cgacgcacgc ccatcgctaa ttggccaggg tgcagttgtc ttgtcttgtt gggagctccc
     8161 aaccaagcgc atttgcaatc aaaaatgcga cgccacgacg ccaaacccaa gaggctgata
     8221 tcatgagccg caaagacgca atcgatactt tgttcctcaa gaagcaacct gcgaccgata
     8281 gagcagcagt cgacaagtcg accgctcgtg ttcgtaccgg agcgatttcg gccatgggtt
     8341 cgtctttgca agagatggct gagggcgcaa aggctgcagc tcggctgcag gatcaactgg
     8401 ctacaggcga agccgtcgtg tccctggatc catccatgat cgacgggtcg ccgatcgcgg
     8461 atcggctgcc ctcagacgtg gatccgaaat tcgagcagct tgaggcgagc atttcgcagg
     8521 aggggcagca ggtgccggtt cttgtcagac cgcaccctga ggctgccggt cgatatcaga
     8581 tcgtatatgg aaggcggcgg ctgcgcgcgg cagtaaatct gcggagagag gtttctgcca
     8641 ttgttcgaaa tctcacggac tgtgaactgg tcgtggccca gggccgcgaa aatcttaacc
     8701 gtgctgacct ctcgttcatt gagaaggctc tcttcgccct gcgcctcgaa gatgcgggtt
     8761 ttgatagagc caccatcatt gccgcgctat ccactgacaa ggccgacctc agccgctaca
     8821 taactgtagc aaggggcata ccgctgaacc tcgccacaca aatcggccca gcgtcgaaag
     8881 cgggtcgatc gcgttgggtc gcacttgccg aggggcttgg gaagcctaag gcaacggacg
     8941 caatcgaagc gatgcttggg tcagagcagt tcaagcaatc tgatagcgat acccgcttta
     9001 acctcatttt caacgccgtt tcaaggccac ctgcgaagac tccaaaaaag gtaagggcct
     9061 ggagcacgcc aaaggggaaa aaggcagcga cgatccgaca agaaactgga cgaacggcgc
     9121 tggttttcga cgagagactg gtgccaactt ttggcgaata tgtcgctgac cagttggaca
     9181 gtctgtacgc ccagttcatt gaaaccaacg gaggaggcaa gctcgaccaa tagtcagggt
     9241 ttcatccaat ttaaagctcc gctcgactga gatggactgg ctctcaccgc aaaagaaaaa
     9301 ggcccccgaa acggcgttcc ggaagacctt ctctgtagtc tcgcagctaa gagaatcgca
     9361 tttccaggaa tcgtagtcaa gggtcccgta agggaaagcg tcatttcgac gggcggattt
     9421 caattgccta acaaaaggta aaaggaaatg cagacgcata tctcaacgac gtcctttggg
     9481 cggcggccga tgacactcgg ccatattgca agccagatgg cagcaaaagc ggtcgcatca
     9541 gacactgtcg cccacaaatg gcaggtcttc cagcacatcc gtgaatcccg gggactgatc
     9601 ggagccacgg accgctcact ctcgatcctg aacgcgctgt tgacgtttta cccggagacc
     9661 gccttgactg gtggtgccga actggtcgta tggccttcta acgaacagct gatggctcgc
     9721 gccaacggca tgcccgccac gacactgcgc cggcatcttg ccatactggt tgattgcggg
     9781 ctcatcattc gccgcgacag ccccaatggc aagcggttcg cccgcaaggg aaggggaggg
     9841 gagattgagc aggcctatgg gttcgatctg tcgccgatcg tcgcgcgggc cgaggagttc
     9901 cgagatctgg cccagacagt gcaagctgaa aaaaaggcct tccgggtggc caaggagcgc
     9961 ttgactcttc ttcgtcgtga cattgtcaaa atgatcgaaa ctggcgtcga agagagcgtt
    10021 cctggaaact ggggaagagt tacccagacc tatcagggga tcatcggccg cctgccacgc
    10081 tcggcacctc ggcagcttgt cgagagtatt gggcaagagc ttcaggaact ttgcatcgag
    10141 atccgtgacg tattggaatc tttcacaaaa acgatgaatc tggacgccaa tgagtcccat
    10201 atcggtcgcc acaaacagaa ttcaaatcca gactctaaat ttgaatctga atacggctct
    10261 ggaaaaaaag atgaagcggg cggcagcgtt gcggaaaccg acaatgtacg gagcttgccg
    10321 aaacgcgagc tgcctttggg aatcgtgctg gatgcctgcc ccgaaatgcg ggaattggcc
    10381 cagggaggtc caattcgaca ttggcgcgac ttgctggcgg cggctgagct tgcccggccg
    10441 atgctgggga ttagtccgag cgcctggcgg gaggcccgcg aaaccatggg cgagcaacac
    10501 gcggcgatca cgctggcttc gatctatcag cgggccggtc agatcaataa cgctgggggc
    10561 tatctgcgca gcctgaccga ccgggccaag gatgggaagt tttcgacctg gccgatggtc
    10621 atggcgttgc tccgggcgaa gctggacgag cagaagaatg cagtcggcgc tggaaagccg
    10681 cgaactgctg aggaggtcga ggatgacagc cgcctccacg tatcggaatc gctgctcaaa
    10741 aacctgcgaa agccgagatc ttggtgatcc tctcgctatt cagccgcggc gatgtcgacg
    10801 tcggtgatca acccggaacg tcgggcgcgg tcgatcaggt tcttgacgga cgagggcgcc
    10861 catttggatc caccgcgcgg cgtgcgctcg tgcagtcttt caagctggcc ggcgatctcg
    10921 cggagcttca ggtcggggtt cgaagaatgg atgccggcca caagcgtcat caggcgatct
    10981 tcgggaagac ggggaggaga ttttttcagg agcgctgcat ccaccaggcg ttccgtcacc
    11041 atccacttca cggcgcggcg aagacgttcc ggcgtccagt cgaagccccg ctgcttgagc
    11101 attcgggcga tgtcgtccca tgtgtgatcc ggtcgcatgc gacgaacggt aggaagccat
    11161 tggttcgcgg acgcctgaat cctatcgcca tatgccgctt tctgggccgc ggtcatcctt
    11221 gccagtgcct ccgggcgctt ttcccggatg cccgggttgc cggaaagctt ggcactggcc
    11281 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    11341 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    11401 caacagttgc gcagcctgaa tggcgaatgg cgataagcta gagctagcat ccaatgcggt
    11461 ccgggctccc gcttggatgc gttcgacgct ggtctcgatc gctaacgcaa tgccgagtga
    11521 gatagatgga taaccatgcg aatgtatcca tcaagctgat aggaaagaat ttgctctcgt
    11581 tgcagtctca aatgttggga actgttgctc cgttcaaatt gcttttcgct atggcgatca
    11641 ttaaactgga ttttaaccag ttctttggga gaggcgtcta tctctcgaaa gtgccaaaaa
    11701 gtcaccgcga gagggacacg atgacgcaaa tgtggaccat tcaatcagaa ggagctcgat
    11761 ctatgaaccg agctcagtcg tcatgaccac ggcgcagcag ttgatctcgc tgctgaagag
    11821 ccacgtcgaa ggcgacgacg agcagttcct gacgattgca ttgcaggctg cagcaagtga
    11881 ggcccgcagg ggtcatggaa aggtggctgt gcaactccgc gagcttgtcg atgcagcgcg
    11941 cgccaacaag gatcgtacct ccgggcgcaa gaaagcccct gttcctattg cgcagccacg
    12001 cggtgatctc gcgaacctgg ttgcggtgcg ctacactgac gttcggttgt cgagcatgat
    12061 ccttcctgcg gagctcgaag ctcgtttgaa acgagtcatt ctcgagcagc gacaacagca
    12121 taagcttcgc agccatgggc tggccccacg gcgcaaactg ttgctcatcg gtccccccgg
    12181 atccggaaag acaatgaccg cggctgcgct cgccggtgaa ctccatacgc cgttgttcac
    12241 cgtgcagctc gatggcttga tgacgaagtt catgggggag accgcctcaa agttgcggct
    12301 ggtgttctcg gcgatggctg aaaccaaagg cgtctacttc ttcgacgagt tcgatgcgat
    12361 cggcgcgaag cgcgcggaac gcaatgacgt gggcgagatc cggcgtgtcc tcaattcgtt
    12421 ccttcaattc ctcgaggagg atgagagtga cggtctggtg gtggcggcga ctaatcaccc
    12481 ggaattgctc gatccagcgc tctttcggcg gttcgatgac gtcatcgagt atgcattgcc
    12541 gagcgttgaa gtcgcgaccg gcattttgca agcgcgccta tccggcttcg acacccagga
    12601 tatcgactgg acaaaagcgg ttgcagatgc tgccggtttg agccaggccg agattgcccg
    12661 cgctgcggcc gacgccgcga aactcgtcgt cctgtccgac cgggaccgca tcacccagaa
    12721 cgatctgtcc ctcgcgatag ccgaacgcaa aggagcggcg tcgcaataat cgatggcaga
    12781 agaaccaccg cgtccactcc cacacgttta tttgcccggt cacgggaaca tccaggacta
    12841 cactgccatc tagcttcacg c
//
